Evaluation of the Habitual Amaranth ( Amaranthus Spp ) Consumption Effect on Biochemical and Anthropometric Indicators of a Women’s Group with Diabetes Mellitus 2 in Michoacán, México

Diabetes Mellitus 2 is a multifactorial disease whose global and national dimensions require a multidisciplinary approach for its control, with nutrition being a fundamental part of this within a healthy lifestyle. The study’s objective was to evaluate the effect of amaranth as a functional food in a group of women with Diabetes Mellitus 2 (DM2) in Michoacán, Mexico over a year, previously with their informed consent. It was of a longitudinal, prospective, and intervention type. Among the comparative results of the beginning and end of the study, it is worth noting that the average blood glucose level decreased 29.86mg / d (118.9 / 89.04); there was an increase in high- density lipoprotein; moreover, the patients did not increase their body weight. The results suggest that amaranth as a food of habitual consumption represents an alternative of health and nutrition for the patient with DM2.


Introduction
Diabetes mellitus 2 is a metabolic, chronic, and systemic disease of multifactorial origin, caused by an alteration in insulin production, use, or action. The diabetes risk factors cover advanced age, ethnic minorities, generalized obesity, adiposity, and socioeconomic status [1]. Type 2 Diabetes mellitus (DM2) occurs at any age, generally over 40 years of age, increasing its risk through obesity, lack of physical activity, dyslipidemias, family history of gestational diabetes, hypertension, and age. In most cases, modifying the lifestyle can avoid the disease. The predominance of diabetes is higher in developed countries. However, its proportional increase is higher in developing countries [2]. DM2 is a chronic disease of great epidemiological interest due to its strong relationship with society's progress. The disease prevalence and incidence have been rising parallel with obesity, sedentary lifestyle, and age [3]. Its primary manifestation is chronic hyperglycemia, which affects the intermediate metabolism of carbohydrates, proteins, and fats [4]. Diabetes is one of the world's leading health problems, and it is considerably affected by excessive body weight and physical inactivity [5]. This pathology has positioned itself in recent decades as a fundamental cause of premature morbidity and mortality worldwide [4]. It is essential to notice that the pathology mentioned above without adequate control leads to a series of micro and macrovascular comorbidities that significantly affect the patient's quality of life and life expectancy. On the other hand, specialists diagnose more frequently DM2 and, in most cases, under advanced stages of late complications [6].
It is worth noting that a component of the treatment of this pathology involves lifestyle modifications, including diet and physical activity, and medication, considering diet modification as the fundamental pillar of its treatment [7]. Several studies have shown that interventions based on diet and exercise reduce the risk of developing DM, are helpful for its treatment, and support the delay of its complications by reducing the abdominal circumference, improving insulin sensitivity, decreasing the plasma glucose and triglyceride concentrations, as well as increased HDL8 values [8].
Over more than three decades, DM2 has gradually formed a lousy panorama in Mexico's public health, frequently associated with obesity and a sedentary lifestyle. The Ministry of Health considers diabetes as the most significant health challenge. This statement represents a clear example of the epidemiological and health care transition that the country is experiencing nowadays. Our country ranks worldwide sixth with people with diabetes [9] (IFD, 2013).
During the past few decades, the number of people who have diabetes in Mexico has increased, and it is currently among the leading causes of death in the country [9]. The data identify 6.4 million Mexican adults with diabetes, signifying 9.2% of adults in Mexico have already received a diabetes diagnosis [10]. The national strategy for preventing and controlling diabetes mellitus is creating indicators to improve eating habits and exercise.
However, mortality has increased by 17% since its implementation [11]. In Mexico, the average age of people who died from diabetes in 2010 was 66.7 years, suggesting a reduction of 10 years of life expectancy; thus, the seriousness of the diabetes epidemic and the fact that it is a preventable disease call for strengthening strategies to deal with it. Its impact on the quality of life of people who suffer from it makes this disease a priority for the health sector [12].
In Michoacán, according to the Ministry of Health's data [13] Amaranth has a high agri-food potential since its seed, plant, and leaves have nutritional values that surpass some commonly used cereals [14]. The analyzes indicate that the contents of crude proteins, lipids, fiber, and ashes of amaranth, in general, are higher than those of grains, while the proportion of carbohydrates is lower.
Amaranth seeds contain around 13 to 18% crude protein, although some authors have found 15 to 22% values. Amaranth proteins are mainly found in the embryo (65%), unlike corn, rice, or soybeans, with 80% of their proteins in the endosperm [15]. Amaranth Amaranth, known as kiwicha in some Central and South American countries, has already achieved a renowned and marked interest along with other grains considered "super grains," such as quinoa, chia, millet, and sorghum, these as an alternative in combating food security and rural development problems [14]. From the perspective of its potential as a functional food, amaranth contains several potentially essential agents in reducing blood cholesterol, such as dietary fiber, squalene, tocotrienols -which are forms of vitamin E-and isoprenoids [16]. Dietary fiber contains indigestible polysaccharides, oligosaccharides, lignin, and substances associated with the plant. The calcium, magnesium boron, iron, and aluminum content are higher than in the seeds of other cereals. Among the most representative bioactive peptides are the Angiotensin-Converting Enzyme (ACE) and Dipeptidyl Peptidase IV Enzyme (DPP IV) inhibitors. DPP IV is responsible for the degradation of insulinotropic hormones related to stimulating the synthesis and promoting insulin secretion by the pancreas' beta cells, thus lowering blood glucose levels [17]. In the last decades, amaranth has been of great importance due to the functional properties of its macromolecules, mainly proteins. phospholipids [19]. Studies in rats fed with diets high in cholesterol demonstrate the positive effect of amaranth consumption on plasma lipid profiles [20]. In contrast, the cholesterol-lowering effects in hamsters produced by consuming protein, whole grain, and amaranth oil isolates [21]. To summarize, apart from its high nutritional value, amaranth is a source of bioactive peptides and a series of phytochemical compounds with beneficial properties for human health and with potential biotechnological development [22]. Moreover, the food product's multiple nutritional and health-benefit properties from the culinary perspective have great versatility for agribusiness and culinary preparations at the domestic level.

Methodology
The research was conducted among 32 women with DM2, with an average age of 68 years, belonging to the Grupo de Diabéticos

Results and Discussion
Depicted below are some general results observed during the study. It is important to remark that there was a shortage of amaranth during December, which impacted the biochemical data of the patients (Chart 1). The decrease in glucose levels is observed in the patients, being more significant during the blood glucose level measurements and the first four months of the amaranth intake regularly (Chart 2). This biochemical indicator increases throughout the study, attributed to the character of amaranth as a cereal, remembering that the usual diet of the patients did not undergo any modification (Chart 3). This indicator behavior in the patients significantly decreased between blood cholesterol samples at the beginning of the study and the first monitoring four months later, with total cholesterol increasing in the following two evaluation periods. However, to better understand this indicator was closely monitored with a lipid profile to characterize the cholesterol type (Chart 4). Throughout characterization of the cholesterol type found in the patients, the study showed a significant increase in HDL between six months and one year after the dietary intervention (Chart 5). Between six months and 12 months after the intervention with amaranth, the research registered an increase in LDL cholesterol levels. Although there was no significant weight loss, this component of body composition remained stable throughout the entire study group. Therefore the Body Mass Index prevailed stable (Table 1). About this indicator, the women in the study had a slight decrease in their hip circumference (Table 2). According to the results, the patients' blood levels over a year evidenced a decrease in glucose between the initial and final states. Regarding triglycerides, these had an upward behavior in the patients. Concerning total cholesterol, this indicator presented fluctuations, and the initial and final evaluations were practically the same. The determinations of the lipid profile, the HDL of the patients rose significantly; LDL also showed an increase, although in a lesser proportion than HDL. About the levels of VLDL cholesterol, they remained without considerable change. Finally, the patients' body weight did not increase. To summarize, Table 3 depicts the different variables that were analyzed using the Friedman test.
This table shows the initial and final behavior of the parameters that were significant throughout the study: glucose, triglyceride, and cholesterol levels, as well as the HDL, LDL, and VLDL levels of the patients. Meanwhile, Table 4 shows that both cholesterol and VLDL did not change their levels between the initial and final states (Tables 5 & 6). On the other hand, the triglycerides, HDL, and LDL increased significantly compared to the decreased glucose level.    Note: 1 The level of the final evaluation is lower than that of the initial assessment. 2 The level of the final evaluation is higher than that of the initial assessment.
*The number in parentheses refers to Mean rank values.

Conclusion
The study evidenced that the habitual consumption of amaranth has a positive effect determined from the amaranth active protein fraction with its inhibitory capacity of DPP-IV related to insulin secretion and the soluble fiber present in amaranth for glycemic control. The presence of polyunsaturated fats allowed an increase in HDL levels. Although the study identified that the amaranth's behavior could be considered a functional food of habitual consumption for the DM2 patients, further studies among a more significant number of individuals will allow emitting that this cereal contributes to improving their quality of life.