Effects of the BICOM Optima Mobile Bioresonance Device on Cell Metabolism and Oxidative Burst of Inflammation-Mediating Cells

Background: Neutrophils are the most common type of granulocyte, a type of white blood cell, found in most mammals. They play an essential part of the innate immune system and as inflammation-mediating cells. The holistic bio resonance method uses the electromagnetic waves it receives from the patient and alters the energy field of the organism. Thus, it can be used as a diagnostic and therapeutic method. Prompted by this background we investigated whether the treatment with the BICOM optima mobile bioresonance device could achieve an anti-inflammatory response of cultivated functional neutrophils by reducing the metabolic activity and the generation of superoxide anion radicals which might cause local oxidative stress in the inflamed tissue. Material and Methods: Human promyelocytes (HL-60 cells) were differentiated to functional neutrophils over a period of 5 days by the addition of 1.5 vol% dimethyl sulfoxide to the culture medium. During the differentiation process, the cells were treated with the program chain “pathogene Ai” of the BICOM optima mobile bioresonance device on three consecutive days in an external mini-incubator at 37 °C for 180 min each. The controls were incubated for the same time in another external mini-incubator without bio resonance treatment. Finally, the basal cell metabolism and the generation of superoxide anion radicals with and without treatment of the functional neutrophils was examined. Results: Treatment with the BICOM mobile bioresonance device had no significant influence on mitotic activity or cell size of functional neutrophils. Measurement of the basal cell metabolism of the functional neutrophils without triggering an oxidative burst resulted in a statistically significant reduction of the basal cell metabolism by 12.2 ± 4.5 % (mean value ± standard deviation; p ≤ 0.05; Wilcoxon-Mann-Whitney test). In accordance with the reduction of the basal metabolism was the reduction in the generation of superoxide anion radicals after treatment with the BICOM mobile bioresonance device by 17.8 ± 3.1 % (mean value ± standard deviation). When compared with the untreated control, this inhibition of radical formation was also statistically significant (p ≤ 0.01; Wilcoxon-Mann-Whitney test). Conclusion: Bioresonance principles are not really accepted in the treatment of acute and chronic inflammatory processes or in wound healing management in conventional medicine. However, the results of this investigation suggest that bioresonance intervention might be very useful in the treatment of acute and chronic inflammatory processes as a complement to conventional therapeutic approaches. Moreover, a treatment with the BICOM optima mobile bioresonance device might improve and maintain health and well-being. Oxidative

microbial pathogens as an essential part of the innate immune system [1], and (ii) as inflammation-mediating cells after having entered the inflamed tissue. In the case of inflammation, the neutrophils migrate from the blood into the tissue and generate reactive oxygen species, preferably superoxide anion radicals, in a so-called oxidative or respiratory burst [2,3]. Although radicals play an important role in intercellular signal transmission [4,5], an excess of radicals in the tissue causes a local oxidative stress and can no longer be neutralized by the body's own enzymes such as superoxide dismutase and others [6][7][8]. The resulting oxidative stress in the tissue causes modifications in biomolecules such as proteins, lipids and DNA. The resulting cell and tissue damage is an initial step in the pathogenesis of multiple diseases [9][10][11][12]. It is well known that the cells of our body emit and receive electromagnetic signals which allow an intra-and intercellular electromagnetic communication [13,14]. In case of disorders or diseases, this kind of cellular communication is disturbed [15]. The holistic bioresonance method uses the electromagnetic waves it receives from the patient and alters the energy field of the organism.
Thus, it can be used as a diagnostic and therapeutic method to improve well-being and the success in the treatment of various diseases [16][17][18]. In a previous study we have already demonstrated that the BICOM optima mobile bioresonance device is able to promote the wound healing process by a stimulation of the metabolic activity and of cell migration and proliferation of cultured connective tissue fibroblasts [19]. Prompted by this background we investigated in the present study whether the treatment with the BICOM optima mobile bioresonance device could also achieve an anti-inflammatory response of functional neutrophils by reducing metabolic activity and generation of superoxide anion radicals upon activation.

BICOM Bioresonance Device
The BICOM optima mobile bioresonance device equipped with the BICOM power applicator GST71 was kindly provided by

Statistical Analysis
Statistical analysis was performed by using the non-parametric two-tailed Wilcoxon-Mann-Whitney test.  (Figure 2). When calculating the mean value ± standard deviation from 5 independent experiments, a statistically significant reduction of the basal cell metabolism by 12.2 ± 4.5 % (p ≤ 0.05) was obtained. In accordance with the reduction of the basal metabolism of the functional neutrophils was the reduction in the generation of superoxide anion radicals after treatment with the BICOM mobile bio resonance device (Figure 2). The reduction in all 5 independent experiments was 17.8 ± 3.1 % (mean value ± standard deviation). When compared with the untreated control, this inhibition of radical formation was also statistically significant (p ≤ 0.01).

Figure 2:
Reduction of the basal metabolic activity (green data points) and radical generation (red data points) of functional neutrophils after daily treatment for three times with the BICOM optima mobile bioresonance device during the 5-day differentiation process. The results of the 5 independent experiments (V512, V530, V545, V571 and V572) are shown as well as the mean of all experiments in the form of the dashed line (green for metabolic activity and red for superoxide anion radical generation). The untreated controls are set as "0" in each experiment.
Although the bioresonance principles are not really accepted in conventional medicine as a method for diagnosis and therapy, the present investigation has shown that bioresonance treatment obviously has a definite effect on the cellular level. One might argue that cell cultures are not similar to the complexity of the human body, but it should be considered that cell cultures allow to focus on selected aspects of living matter. The investigation presented here uses functional neutrophils ± treatment with the BICOM optima mobile bioresonance device and has its focus on two principle characteristics of these cells: (i) the metabolic activity and (ii) the generation of superoxide anion radicals in the course of an induced oxidative burst. In a previous investigation we found that treatment of connective tissue fibroblasts with the BICOM optima mobile bioresonance device resulted in a stimulation of basal cell metabolism and consequently in an improved wound healing [19].
At first sight, the finding of this present study, namely the reduction in metabolic activity and generation of superoxide anion radicals of functional neutrophils, might be somehow contradictory. However, chronical inflammatory and complicated wound healing processes are also associated with the occurrence of an excess of oxygen radicals which cause a local oxidative stress in the tissue [25][26][27].

Conclusion
Bioresonance principles are not really accepted in the treatment of acute and chronic inflammatory processes or in wound healing management in conventional medicine. The results presented here suggest that bioresonance intervention might be very useful in the treatment of acute and chronic inflammatory processes as a complement to conventional therapeutic approaches. Moreover, a treatment with the BICOM optima mobile bioresonance device might improve and maintain health and well-being.