In Vitro Phytochemical Screening, Anti-Inflammatory and In Vivo Anti-Depressant, Anxiolytic Activities of Methanolic Extract of Leea aequata (leaves)

Anti-Depressant, Anxiolytic Activities of Methanolic Extract of Leea aequata (leaves). The core aim of the current research study was expose the in-vitro Anti-Inflammatory along with in-vivo Anxiolytic actions of methanolic extract of the aequata Leaves under the family of Vitaceae. Leaves of the plant Leea aequata were collected from KEPZ Hill tract area, KEPZ, Chittagong, Bangladesh in the month of January 2018. This has been created that plants which also naturally synthesis as well as collect some of the secondary metabolites such as alkaloids, volatiles oils,

They comprise a host of well-known organisms together with trees, grasses, forbs, shrubs, vines, ferns in addition to mosses [1].
Traditionally the word plant implies that a taxon with properties of multicellularity, cell structure along with walls holding cellulose in addition to organisms competent of photosynthesis [2]. The plant Leea aequata is usually used in tribes regions for Astringent, Antipyretic, Anthelmintic, Alexiretic [3]. This is also helpful in Bronchitis, Dyspepsia, Anaesthesis of the skin, Bilious fever, Itching, Leprosy, Tuberculous and Ulcer. This is also recognized as a Kakjangha in the Bengali [4]. As it is known that inflammation is the complex method which is normally associated with the pain and involves incidences like the raise of the vascular permeability, raise of the protein denaturation in addition to membrane alteration [5].
Cells in body are harmed by microbes, chemical agents, physical agents, the damage is in form stress. Inflammation of the tissue is because of reply to stress [6]. Loss of task happens depends on site in addition to extent of the injury [7]. In view of the fact that inflammation is one of body's nonspecific inner systems of resistance, the reaction of the tissue to accidental hack is analogous to the reply that consequences from other sorts of tissue injure, reasoned by burns because of heat, radiation, viral or bacterial attack [8]. The duration of an nervous feeling can for a while be out of the proportion to original trigger, Physical symptoms like enlarged blood pressure in addition to nausea, can also happen to evident [9]. These answers move further than anxiety into anxiety disorder. So anxiety disorders happen when a result is out of the proportion to what might usually be supposed in a condition [10].
The main aim of the present research work was find out the In-     (Table-3

Animals
Mice weighing about 28-32 gm aged about 1.5 month were purchased from animal house of Department of Pharmacy, Jahangirnagar University, Savar, Dhaka, Bangladesh. All the animals were acclimatized to new environment for a period of one week.
During the test period the animals were kept in a well ventilated animal house at 25 0 C. They were supplied with standard pellets and fresh drinking water. All the mice were kept in cage and maintained with natural 12h light and dark cycle.

Egg Albumin Denaturation Method
The anti-anti-inflammatory activity of methanolic extract of a plant was studied using the following procedure was followed by many authors [11] for evaluating the Percentage of inhibition of protein denaturation ( Figure 1). The reaction combination was consisting of trial extract at unusual concentrations. pH of reaction combination was regulated using small quantity of 1N HCl. The trials were hatched at the 37ºC for 20 min. in addition to heated for 57ºC for 2 min. Later than cooling the trials, turbidity was determined by spectrophotometrically at the 416 nm [12]. The test was done triplicate. The percentage (%) inhibition of the protein denaturations were considered as follows:

Phytochemical Screening
The qualitative phytochemical screening was performed to ensure the presence or absence of secondary plant metabolites. In our investigation we have found positive results for carbohydrates, Alkaloids, Phytosterol, Phenol, Flavonoids, Tannins and Saponins.
The result showed in (Table 3).

In-vitro Anti-inflammatory Activity
The plant extract inhibited the protein denaturation by from a lowest 5% when conc. was 125 µg/ml. To the highest % of inhibition was 35% when concentration was 1000 µg/ml as compared to standard drug diclofenac Na inhibited by 82% at 1000 µg/ml.

Anti-Depressant Activity
Test of Tail

Conclusion
In conclusion, this well-informed study evaluated significant phytochemical screening (Table 1)  If possible more precise studies are needed to elucidate their mechanism of actions for individual photochemical. MELA=Methanolic Extract of Leea aequata. MELA=Methanolic Extract of Leea aequata. MELA=Methanolic Extract of Leea aequata.