Serological-Diagnosis for Zoonotic-Disease Pediatric-Brucellosis by Gold-Standard Affecting Community-Pediatric-Health at High-Altitude Area, Taif, Ksa

Brucellosis is a significant ZD causes by B., it has several species, and are differ in virulence, more famous in KSA are B. (melitensis and abortus) [1]. Reservoirs of B. melitensis is in (sheep, goat and camel) and B. abortus is in (cattle) [2]. That causing an economic burden in animal host, which present in unpasteurized raw milk and its dairy products [3]. Human got infections by inhalation, handling animals or animal carcasses such as meat, blood, urine, vaginal secretions, placenta, and fetus [4].

This paper was for discharge of "Serological-Diagnosis (SD) for Zoonotic-Disease (ZD) Pediatric-Brucellosis (PB) by Gold-Standard (GS) Affecting Community-Pediatric-Health (CPH) at High-Altitude (HA) Area, Taif, KSA", the negative infections by Brucella (B.) abortus was 85.5%, and B. melitensis was 81.7%. Positive infections by B. abortus was 14.5%, less than by B. melitensis was 18.4%. The 1/80 titer difference between negative B. abortus than B. melitensis was about 4%. In the positive, the difference was found in the titer 1/160 were about 1.2%, 1/320 was 0.3% and 1/640 was 2.3% respectively. Titer 1/640 indicated the disparity of infected cases from the old; the patient had old infection and was the highest IgG. Recent infection was less than the indication of the preventive measures used to reduce the common diseases, including veterinary care for farms and the quality of manufacturing milk and milk products Lack of modern infections may be ways to protect a CPH from ZD as well as to protect livestock from infection. Concluded from that, the ZD as BP can be easily spread from animals to children through milk and its product. As well, the animal as sources of infection is too hard for CPH. Management of milk product can help in happened of ZD as PB and affecting CPH. Recommended for Vet. Observation must be clear for farms, milk and its product must not use from "House Made", that all for protect CPH at Taif HA area. The aims of this work were for study SD for ZD for PB by GS to deliver preliminary opinion material and the first signs about an extent at HA area, Taif, KSA. As well was considered affecting CPH.
This study will be the firstly report the incidence of ZD as PB by GS at Taif HA area, KSA.

Methodology
Research Area: Taif HA area was studied for the collection of blood samples from private hospitals after had approvals from hospitals and children parents. That was with condition did not record any personal or family information [14].
Sample Collections: Blood sample (5ml) were collected, serum was separated and were stored at fridge, until tested for the presence of B. IgG [15].

Serological Diagnosis (SD): Detection of IgG by Rose Bengal
Plate Test (RBPT) [16], so that were gave positive or negative as start for diagnosis. If it was positive must made Serum Agglutination Test (SAT) titration for B. (abortus and melitensis) [17]. If it is 1/80 and below will consider negative, and more than 1/80 will consider positive [17].
Data Analysis: It was used "Simple Basic Excel Formulas" for management data for result in tables and diagram [18]. Table 1 and Graph 1 showed mean prevalence of total SD results, which showed RBPT detection of IgG, the negative infections by B.

*SD: Zoonotic-disease, *B.: Brucella
The KSA society had the livestock of (sheep, goats and camels), which was the highest than of cows where it was a source and a reservoir of B. melitensis, which was definitely the main source of infection. As well, this was an evident from the positive infection by
Milk and products were present in the "Municipal Markets" in Taif as HA Area and may be prepared locally at house (House Made). That observed bacteria have not eliminated well in a healthy way. That the home manufacturing was incomplete or good. So, eating milk or products could lead to the occurrence of infection, especially for children as ZD and a common disease, so that would affect CPH [7][8][9][10][11][12][13].

Conclusion
ZD as BP can be easily spread from animals to children through milk and its product. As well, the animal as sources of infection is too hard for CPH at Ha area. Management of milk product can help in happened of ZD as PB and affecting CPH.

Recommendation
Vet. Observation must be clear for farms, milk and its product must not use from "House Made", that all for protect CPH at Taif HA area.