Irreversible Dilated Cardiomyopathy After Abuse of Anabolic Androgenic Steroids: A Case Report and Literature Review

Numerous athletes make use of anabolic-androgenic steroids (AASs), the synthetic derivatives of the male hormone testosterone, to increase and strengthen muscle mass, even if this is illegal but impossible to control. The effect of abuse of these hormones on the body and in particular on the heart is still not entirely known. These substances can produce adverse effects on various organs, and in literature several cases of cardiac disease have been reported in athletes after previous abuse of AASs [1-13] documenting dilated cardiomyopathy (DCM). However only few of them have shown irreversible DCM [1,6,8,13]. We report the case of a 31-yearold white male, bodybuilder, who had previously used AASs. He presented to our attention with dilated cardiomyopathy and severe irreversible left ventricular systolic dysfunction. We focused on the mechanism on AASs induced irreversible LV dysfunction.


Introduction
Numerous athletes make use of anabolic-androgenic steroids (AASs), the synthetic derivatives of the male hormone testosterone, to increase and strengthen muscle mass, even if this is illegal but impossible to control. The effect of abuse of these hormones on the body and in particular on the heart is still not entirely known. These substances can produce adverse effects on various organs, and in literature several cases of cardiac disease have been reported in athletes after previous abuse of AASs [1][2][3][4][5][6][7][8][9][10][11][12][13] documenting dilated cardiomyopathy (DCM). However only few of them have shown irreversible DCM [1,6,8,13]. We report the case of a 31-yearold white male, bodybuilder, who had previously used AASs. He presented to our attention with dilated cardiomyopathy and severe irreversible left ventricular systolic dysfunction. We focused on the mechanism on AASs induced irreversible LV dysfunction.

Case Report
The patient was a 31-year-old man, bodybuilder, with no family history of cardiac disease and no cardiovascular risk factors. When the patient came to our attention, he had received a diagnosis of dilated cardiomyopathy (undocumented) about one year before and started treatment with beta-blockers and diuretics; he then spontaneously suspended the therapy after a few months. He admitted having previously taken high doses of various AASs to increase his muscle mass, including stanozolol, boldenonem, Illicit usage in athletes of androgenic anabolic steroids, such as testosterone and its derivatives, can cause myocardial dysfunction, that is usually reversible after the end of the abuse. However, in some cases myocardial dysfunction can be severe and irreversible for reasons that are not clear. We report the case of a 31-year-old patient, bodybuilder, who came to our attention for acute heart failure secondary to a dilated cardiomyopathy, with severe and irreversible left ventricular systolic dysfunction (LVEF 23%). Instrumental and laboratory examinations excluded myocarditis and coronary artery disease as a possible etiology of the left ventricular systolic dysfunction. The patient had previously taken different types of androgenic anabolic steroids (testosterone and derivatives), for almost twelve years and also growth hormone (GH) for 1 year, and never stopped hard exercise training throughout in order to improve his performance as a bodybuilder. He had been diagnosed with left ventricular systolic dysfunction, symptomatic for heart failure (NYHA class II-III), about six months after cessation of the abuse and the severe LV dysfunction did not reverse. We believe that the abuse of steroids may be the cause of our patient's left ventricular dysfunction, that became worse and irreversible due to many years of usage, in association with intense physical training and GH; our hypothesis is strengthened by experimental studies in animals and autopsy immunohistochemical studies demonstrating a direct cardiotoxicity of anabolic androgenic steroids . The possible etiologic mechanism underlying acute and chronic cardiotoxicity in humans of these substances in humans warrants closer clinical investigation. trenbolone, testosterone proprionate and enanthate. His abuse of these substances had lasted for twelve years, and he also took human growth hormone for 1 year. He had stopped taking these substances one year before he came to our attention, after the diagnosis of dilated cardiomyopathy. The patient presented at the emergency room of our hospital with worsening dyspnea and orthopnea, symptoms that had started two months before and showed an increasing trend. He was apyretic, blood pressure was 120/80 mmHg, the heart rate was 90 bpm, the respiration rate was 25 breaths/min and peripheral arterial oxygen saturation was 95%.
Physical examination showed a muscular man, the apex beat was not evident, the jugular at baseline showed an absent X' descent with a positive hepato-jugular reflux and heart sounds were regular at the auscultation ; there was also minimal ankle swelling and , bilateral basal crackling rattles were audible at lung auscultation.
The ECG showed a sinus rhythm with a frequency of 88 bpm, signs of left atrial enlargement, a left axial deviation and negative T-waves on D1 and aVL and from V4 to V6; no previous recent ECG was available for comparison. Chest X-ray showed pulmonary          [14,15], is a more profound damage to the myocytes that can be caused by the synergism of AAS, possibly at high dosage and for a prolonged span of time with other factors that enhance the AAS damage, first of all strenuous exercise training. We will examine first the AAS general toxic effect mechanisms on cardiac myocytes and then the AAS synergism with strenous exercise and with other potential myocites damaging factors like the growth hormone (GH).  [16]. The mechanisms underlying this cardiotoxicity are still not properly understood, but some studies suggest that the adverse effects of AASs on cardiac muscle can be direct: in rat cardiomyocytes, AASs induce apoptotic cell death in a dose-dependent manner [17]. The apoptotic process may be triggered by a membrane receptor -second messenger cascades that increase polyamines which are known to mediate uncontrolled transmembrane Ca ++ flux and also by increasing intracellular Ca ++ via Ca ++ influx and Ca ++ mobilization from the sarcoplasmatic reticulum [17].
The uncontrolled increase of Ca ++ affects apoptotic mitochondrial membrane permeability and triggers the release of apoptogenic factors including caspase-9 from damaged mitochondria [17]. This mechanism was confirmed by an in-vitro study on the cardiomyocytes ultrastructure of rodents after AASs exposure demonstrating mitochondria and myofibrils aberrations similar to those observed in the early stages of human heart failure [18].
Post-mortem studies have corroborated the apoptotic mechanism. In a post-mortem immunohistochemical study, histological analyses were performed on heart tissue of two bodybuilders: the analysis showed important myocytes and endothelial cells apoptotic alterations [14], after various years of abuse of methelonone and nandrolone; these body builders also had a history of very strenuous training that could have enhanced these histopathological alterations. The clinical consequence of this myocardial damage has been corroborated in numerous clinical studies. In athletes that routinely used AASs, the onset of a subclinical impairment of both systolic and diastolic myocardial function has been reported even several years after the cessation of the abuse [13] [17].
Then, the loss of cellular energy production causes chronic hypoxia of the cardiac muscle and cell death can ensue due to apoptosis rather than necrosis. In addition, nandrolone could act through blocking or down regulating the mechanism implicated in the improvement in antioxidant defense in animal during exercise as demonstrated in animals [22]. GH is another potential harmful substance when taken at high dosage for long periods of times as our patients did for almost 1 year. Concomitant use of AAS and GH further increased LV mass and associated with concentric remodeling of LV [23].
Although left ventricular contraction is impaired in congenital GH deficiency, long term GH excess leads to cardiac hypertrophy and decreased cardiac function [24]. Our case recalls another literature report of a 41 body-builder with severe and irreversible dilated cardiomyopathy that had taken AAS and GH (actually insulin-like growth factor-1 that is an active metabolite of GH) for long periods of time in association with intense physical training; he did not have any other risk factors for DCM [6] [25]. Our patient did not have dental amalgama fillings [26] but ate fish regularly 1-2 times/week so potential mercury exposure was modest. A hair analysis could be of aid for chronic heavy metal toxicity screening [19]. Our patient also presented abnormal plasma lipoproteins and laboratory signs of hepatotoxicity: these are other possible side effects of anabolic steroids abuse [27]. Echocardiography can have an important role in the detection of subclinical systo-diastolic dysfunction in AASs users and can be a useful way to discourage the use of these substances in sportsmen, revealing initial forms of heart injury through SRI and TDI analysis [20].

Conclusion
Long abuse of AAS along with intense physical exercise and GH conjured up irreversibly damaged cardiac myocytes mainly by activating apoptotic mechanism rather than causing necrosis. The abuse of AAS and GH by athletes to improve their performance needs to be strictly controlled and forbidden in view of the possible onset of serious, potentially irreversible cardiovascular diseases.
The cause-effect relationship between AASs abuse and the onset of heart failure and irreversible cardiotoxicity mechanisms warrant closer investigations.

Author's Contribution
Drs Angelini and Pollice gave the same contribution as first authors.