A Practical Method to Predict the Incidence of Anterior Demodex Blepharitis

Results: The relationship between symptoms and positive infection rate is correlated. Conclusion: Due to the inability of the anterior blepharitis diagnostics to isolate substances produced by the demodex, we constructed a method that provides doctors with a highly plausible clinical testing process. abstract


Introduction
According to Medilexicon's medical dictionary, the prevalence rate of Allergic conjunctivitis is around 10%. The causes include pollen, animal fur, contents of various eye drops, cosmetics, and dust mite tissue. Most of the symptoms can be resolved by the use of medication, however the symptoms of some patients can't be relieved with medicine. One of the reasons is that the reproduction of mites and the rate of allergen creation is not altered by medication and Blepharitis becomes a chronic decease.
Demodex blepharitis generally results from D. folliculorum and D.
brevis [1][2][3]. The former infests the roots of eyelashes, becoming the leading cause of anterior blepharitis. The latter infests the The two bacteria may also have links to skin conditions like rosacea [4]. It can be assumed that waste from the demodex and allergic reactions will cause the amount of patient discharge to increase. The proteins and excreta created by the D. folliculorum can cause itchiness, the fourth type of Allergic immune response Most of the symptoms induced by allergens can be resolved by the use of medication, however the symptoms related to demodex can't be relieved with medicine. The symptoms of inflammation caused by Demodex can vary from none to very diverse and are often hard to cure with medical treatment. The purpose of this study is to find a way to increase the rate of diagnosis, and also to find connections between the four patient symptoms (Foreign body sensation, discharge, itching, and dryness) and the rate of infestation.

Material and Methods:
When patients voluntarily mentioned one of the four symptoms in clinic, the doctor included them as a candidate in this study. The three epilated eyelashes from patients were examined using a slit lamp and a microscope (25 X, Olympus SZX16 model) for identification of demodex.

2/4
on patients with negative results due to being unable to check every eyelash. The purpose of this study is to find a way to increase the rate of diagnosis utilizing the third method, and also to find connections between the four patient symptoms (FBS, discharge, itching, and dryness) and the rate of infestation.

Patient Population and Selection
The study was conducted on 127 adult patients, composed of 29 males and 98 females, ranging from 58 to 96 years old, exhibiting at least one of the four symptoms.

Methods of Data Collection
When patients voluntarily mentioned one of the four symptoms in clinic, the doctor included them as a candidate in this study. The doctor recorded the symptoms of each individual patient assigning a score equally depending on the number of symptoms (score1~4 as blue bar in Figure 1)  If Demodex was discovered, it was identified by its order and the sample number was recorded (Figure 3). If all three samples were clear of demodex, then it was recorded as non-infested.

Figure 3:
The percent of Demodex found in the first and subsequent eyelashes is 52% (Blue area), 39%(Red area) , and 9%( Green area) in that order.

Results
The positive diagnosed rate is 67%, with the average age of 72 years old (85/127). The clinical findings under slit lamp and microscopic findings are shown in figs.1a and 1b. The relationship between symptoms and positive infection rate is shown in Figure   1. The percent of Demodex found in the first and subsequent eyelashes is showed in Figure 3.

Discussion
Clinically, Demodex infestation can cause many ocular diseases, i.e. the destruction of eyelashes, Meibimitis leading to trichiasis or enlarged glands, a hypersensitive reaction inducing lid margin inflammation, an unstable tear film or even superficial corneal vascularization, marginal infiltration, a phlyctenule-like lesion, superficial opacity, and nodular scarring [5,6]. At present, detection and counting of Demodex eggs, larvae and adult mites in epilated lashes under microscopy can make the final diagnosis under Slit-lamp examination (dandruff at the root of eyelashes [7,8] and clinical history (chronic blepharitis which is refractory to conventional treatments). However, a negative finding under microscopy cannot exclude the possibility of Demodex infestation.
One limitation is that we can only see the D. folliculorum on top of the eyelash, and not those inside the sebaceous glands.
There is a strong correlation between a patient's serum reactivity to Bacillus antigen (a bacterium from Demodex 9,10,11) and the presence of ocular rosacea. This is indirect evidence for Demodex infestation [9,10]. Due to the inability of the anterior blepharitis diagnostics to isolate substances produced by the demodex, we constructed a method that provides doctors with a highly plausible clinical testing process. The results of the experiment show that there is a high positive correlation between the symptoms and infestation rate. It is also found that around 90% of the infestations can be identified through the first two eyelashes inspected, but there is still a chance of it not being accurate. If the patient's symptoms and signs are highly suspect for the diagnosis of demodex blepharitis, it is advisable to repeat the process to increase the diagnostic accuracy.

Conclusion
Demodex mite plays an important role in the occurrence of a series of ocular surface diseases such as Demodex blepharitis, meibomian gland dysfunction, conjunctival inflammation, and corneal lesions. Ocular infestation has a close relationship with the systemic infestation. Further studies are needed for developing easy and sensitive diagnostic methods and more effective and specific treating regimens.