Extracts of Dialium Guineense Stem Bark Ameliorates
CCl4-induced Oxidative Stress in Liver of Wistar Rats

Abu OD; Okuo AV; Osemwota OF

doi:10.26717/BJSTR.2022.46.007330

Research ArticleOpen Access

Extracts of Dialium Guineense Stem Bark Ameliorates CCl4-induced Oxidative Stress in Liver of Wistar Rats

Volume 46 - Issue 2

**Abu OD¹*, Okuo AV² and Osemwota OF³**

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- ¹Department of Biochemistry, Faculty of Life Sciences, University of Benin, Nigeria
- ²Department of Chemistry, College of Arts and Sciences, University of Kentucky, USA
- ³Department of Medicine, Faculty of Clinical Sciences, College of Medicine, Lagos State University, Ikeja, Lagos State, Nigeria

Received: May 03, 2022; Published: September 21, 2022

DOI: 10.26717/BJSTR.2022.46.007330

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Abstract

The aim of the present study was to investigate the capacity of extracts of Dialium guineense stem bark to ameliorate carbon tetrachloride (CCl4)-induced oxidative stress in liver of Wistar rats. Adult male Wistar rats (n = 25) weighing 160 – 180 g (mean weight = 170 ± 10g) were randomly assigned to five groups (5 rats per group): normal control, CCl4 control, silymarin, aqueous extract and ethanol extract groups. With the exception of normal control, the rats were exposed to CCl4 at a single oral dose of 1.0mL/kg body weight, bwt. Rats in the silymarin group were administered silymarin (standard hepatoprotective drug) at a dose of 100 mg/kg bwt, while those in the two treatment groups received 1000 mg/kg bwt of aqueous or ethanol extract orally for 28 days. Activities of antioxidant enzymes such as catalase, Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx) and Glutathione Reductase (GR) were evaluated in plasma. The results showed that there were no significant differences in the concentrations of TP among the groups (p > 0.05). The activities of all the antioxidant enzymes measured as well as levels of GSH and NO were significantly lower in CCl4 control group than in normal control group, but they were increased by extract treatment (p < 0.05). However, the level of plasma MDA increased by CCl4 intoxication reduced after treatment (p < 0.05). These results suggest that extracts of D. guineense stem bark could potentiate the antioxidant system in the amelioration of CCl4-induced oxidative stress in rat liver.

Keywords: Antioxidant Enzymes; Dialium Guineense; Liver; Lipid Peroxidation; Oxidative Stress

Abbrevations: GPx: Glutathione Peroxidase; GR: Glutathione Reductase; SOD: Superoxide Dismutase; ROS: Reactive Oxygen Species; MOD: Malondialdehyde