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Research ArticleOpen Access

Electroacupuncture Against Ethanol-Induced Gastric Ulcer in Rats: Effects on Gastric Contractility and in the Levels of TNF-α

Volume 11 - Issue 5

Aline French de Lima Vitorino1*, Loyane Almeida Gama2, Mariana Pirani Rocha2, Luciana Aparecida Corá3, José Ricardo de Arruda Miranda2 and Madileine Francely Américo1

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    • 1Institute of Biological and Health Sciences, Brazil
    • 2Institute of Biosciences, Brazil
    • 3Centre of Health Sciences, Campus Governador Lamenha Filho, Brazil
    • *Corresponding author: Aline French de Lima Vitorino, Madileine Francely Américo, Institute of Biological and Health Sciences, Valdon Varjão Avenue, no. 6.390. CEP: 78600-000 Barra do Garças, Mato Grosso, Brazil

Received: November 28, 2018;   Published: December 06, 2018

DOI: 10.26717/BJSTR.2018.11.002153

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Abstract

Objective: To correlate the effects of the Electroacupuncture (EA) with the levels of TNF-α and the gastric contractility in both ulcerated and non-ulcerated control rats.

Methods: Before the experiments, a magnetic marker was surgically implanted in the gastric serosa of all animals used in this study. Ten days after the surgical procedure, the animals were randomly assigned in groups (n=9 per group) whose received by gavage phosphate buffered saline (non-ulcerated control groups) or ethanol (ulcerated groups). EA treatments were applied every three days during 16 consecutive days in both control (C) and ulcerated (U) groups at sham (S) point (CS and US) and ST-36 acupuncture point (CST-36 and UST-36). Magnetic and electrical gastric activity recordings were also performed. TNF-α was measured by ELISA and all data were statistically analysed by ANOVA.

Results: In the ulcerated animals which received sham EA (US), the frequency and amplitude of contractions were reduced, whereas the levels of TNF-α were increased. EA applied at UST-36 group had significantly increases in the frequency and in the amplitude of contractions. A clear inverse correlation (ρ=0.9) between motility parameters and TNF-α levels in ulcerated rats was observed.

Conclusion: EA in ST-36 had improves the gastric contractility in ulcerated rats by a pathway that involves decreases in the levels of TNF-α.

Keywords : Cytokine; Electroacupuncture; Gastric Contractility; Gastric Ulcer

Abbreviations : GI: Gastrointestinal; TNF-α: Tumor Necrosis Factor-Alpha; EA: Electroacupuncture; EGG: Electrogastrography; ACB: Alternating Current Biosusceptometry; CS: Control Groups at Sham; US: Ulcerated Groups at Sham; FFT: Fast Fourier Transform; RSA: Running Spectrum Analysis; AUC: Area Under Contraction; R: Recordings; ANOVA: Analysis of Variance; SD: Standard Deviation; NSAID’s: Non-Steroidal Anti-Inflammatory Drugs; ICC: Cells of Cajal

Introduction| Methods and Methods| Results| Discussion| Acknowledgement| Authorship Contribution| Financial Support| References|