Electrochemical Characterization for Determination of PNA Surface Density and Rate Electron Transfer Constant Based on Methylene Blue Accumulation Charge

Mohd Hazani Mat Zaid¹ and Jaafar Abdullah^1,2*

Received: November 14, 2018;   Published: November 26, 2018

DOI: 10.26717/BJSTR.2018.11.002082

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Abstract

Here we report the PNA surface density and ratio of EDC/NHS was used as coupling agent in electrochemical application based on methylene blue (MB) accumulation charge. In this study, chronouclometry technique has been used to calculate PNA probe density to obtain optimum PNA surface density for electrochemical sensor application. Furthermore, kinetic behavior of PNA electrochemical biosensor also have been investigated to determine the rate electron transfer of MB before and after hybridization. The obtained data revealed that the PNA bound to modified electrode at optimum probe concentration activated at 5/5 mM of EDC/NHS to give highest electrochemical signal. Meanwhile the MB rate electron transfer was obtained in this study was about 2.9 s−1 before and 2.5 s-1 after hybridization by using laviron approach. These result demonstrated that MB has intercalated into DNA through long range charge transfer.

Keywords : PNA; Electrochemical Biosensor; Methylene Blue

Abbreviations : NHS: N-Hydroxy Succinimide; MB: Methylene Blue; CC: Chronocoulometric; EDC: Ethyl Dimethylaminopropyl Carbodiimide; Ks: Electron Rate Transfer; FTIR: Fourier Transform Infrared Spectroscopy