*Corresponding author:Amin Talebi Bezmin Abadi, Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Received: February 01, 2018; Published: February 15, 2018
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Background and aims: Helicobacter pylori (H. pylori) is a colonizer in the gastric mucosa of more than 50% of the human population. Complications due to this bacterium include chronic gastritis, gastric mucosa-associated lymphoid tissue lymphoma, peptic ulcer and gastric adenocarcinoma. The aim of this study was molecular detection of H. pylori in biopsy samples by amplification of conserved sequences of dupA gene.
Materials and Methods: The in silico study for detection of conserved region in dupA was performed. Seventy-nine biopsy samples were collected and DNA was extracted from them. The polymerase chain reaction (PCR) was performed to amplify dupA conserved region. The glmM gene was also amplified as a standard control alongside the dupA gene.
Results: Results of 79 samples, 31 and 48 were collected from males and female patients, respectively. The age range of patients was 13-85 years; Mean±SD (49.68±18.82).The patients’ complications included 35.4% reflux (13.92% males and 21.52% females), 32.9% gastritis (7.59 males and 25.31% females) and 8.9% peptic ulcer (2.58 males and 6.32% females). The PCR amplification of dupA showed 98% and 100% sensitivity and specificity, respectively, whereas results for glmM were 26% and 100%, respectively.
Conclusion: The findings exhibited that the amplification of dupA conserved region with a high sensitivity and specificity can be considered as a rapid diagnostic method for H. pylroi identification in biopsy specimens.
Keywords: Helicobacter pylori; biopsy samples; Diagnosis; dupA gene; glmM gene
Abbreviations: RUT: Rapid Urease Test; UBT: Urea Breath Test; SAT: Stool Antigen Test